MT-PCR - A rapid, reliable and effective tool for assessing toxic 'algal' blooms in Victorian water supplies

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Description

This project addressed a need for a sensitive, accurate and reliable testing method to aid assessment of the toxicity of algal blooms and assist water management. Increasingly, diagnostic dilemmas are resolved through the use of DNA-based technologies which often provide high sensitivity and specificity and are efficient both in terms of costs and time. However, to date, no such test was available to the Victorian water industries. This project sought to bridge this gap by developing an automated DNA-based diagnostic assay for cyanobacterial bloom assessment blooms in Victorian waters. The assay exceeds expectation in its ability to accurately quantify levels of toxigenic cyanobacteria in bloom samples, retains exceptionally high specificity and sensitivity and each assay out-performs common conventional PCR approaches established in the literature. Four toxigen assays (microcystin, nodularin, cylindrospermopsin and saxitoxin) were designed, tested and optimised. This book is co-published with Water Research Australia

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Publisher -

Author(s) - Aaron Jex

Paperback

Published Date -

ISBN - 9781780407555

Dimensions - 29.7 x 21 x 0.2 cm

Page Count - 31

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