The use of animal, including human, cell culture has expanded enormously during the last 25 years, with new applications appearing all the time. The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basic techniques. Revised and updated chapters cover topics such as equipping a cell culture laboratory, sterilization, culture media, basic culture technique, cell cloning, the isolation of new cell lines, contamination procedures, and good laboratory practice. There is a brand new chapter on the microscopy of living cells, and an appendix of online resources for cell biology.